Protocol 32 powder dispersing in a glass of water

Protocol 32

Thirty-two actives.

Every dose published. Every rupee printed. The strongest human trial behind each one.

  • 32 Actives
  • 8.5 G One sachet
  • ₹38.30 Raw material

Thirty two actives.8.5 grams.One sachet a day.

  • 01

    Creatine Monohydrate

    1,500 mg

    Minimum 99.9% purity

    • ENERGY
    • BRAIN
    ₹1.20 a sachet

    Why it is here

    The instant battery, with magnesium. Creatine holds the charge your muscle and brain draw on in the first seconds of any effort, and vegetarians carry less of it than anyone.

    The trial

    Avgerinos 2018, Experimental Gerontology. Pooled trials: better short term memory and reasoning in healthy adults.

  • 02

    Algal DHA

    750 mg beadlet, 300 mg DHA

    Water soluble, 40% DHA

    • BRAIN
    • STRESS
    • JOINTS
    • EYES
    • HEART
    • LIVER
    ₹10.50 a sachet

    Why it is here

    The link the Oxford trial turned on. DHA is what brain cell membranes are largely built from, it keeps receptors soft enough for signals to land, and it helps the liver ship fat out rather than store it. On an Indian vegetarian plate it is close to absent.

    The trial

    Yurko-Mauro 2010, Alzheimer's and Dementia. 900 mg of DHA a day for six months improved memory and learning in healthy adults over 55.

  • 03

    Glycine

    700 mg

    Free form amino acid, minimum 99%

    • STRESS
    • SKIN
    • DIGESTION
    • IMMUNITY
    • JOINTS
    • HEART
    • LIVER
    ₹0.25 a sachet

    Why it is here

    Every third link in a collagen strand is glycine, and it is one of the two building blocks of glutathione, your repair antioxidant. Two chains, one amino acid.

    The trial

    Bannai 2012, Journal of Pharmacological Sciences. Glycine before bed improved sleep quality and cut next day fatigue.

  • 04

    Magnesium Carbonate

    695 mg, 174 mg magnesium

    25% elemental

    • ENERGY
    • BRAIN
    • STRESS
    • IMMUNITY
    • HEART
    ₹0.15 a sachet

    Why it is here

    It releases the charge creatine holds, and the enzymes that switch vitamin D into its active form need it.

    The trial

    Abbasi 2012, Journal of Research in Medical Sciences. Eight weeks of magnesium improved insomnia severity, sleep time and sleep efficiency in older adults.

    Carbonate, because chelated forms such as magnesium bisglycinate are not yet on the FSSAI permitted list for this category.

  • 05

    TMG

    500 mg

    Betaine anhydrous, trimethylglycine, minimum 99%

    • BRAIN
    • HEART
    • LIVER
    ₹0.35 a sachet

    Why it is here

    The second route for clearing homocysteine, and a spare for choline in packaging fat out of the liver.

    The trial

    Olthof 2003, Journal of Nutrition. Betaine lowered fasting homocysteine in healthy men and women within two weeks and held it for six.

  • 06

    Choline Bitartrate

    500 mg, about 200 mg choline

    Coated, minimum 97%

    • BRAIN
    • STRESS
    • HEART
    • LIVER
    ₹0.45 a sachet

    Why it is here

    Choline is what one of the brain's main messengers is made of, what your body makes TMG from, and the packaging fat needs to leave the liver. Eggs are the main food source, and most Indian plates do not have them.

    The trial

    Fischer 2007, American Journal of Clinical Nutrition. Taken off choline, most healthy men developed fatty liver or muscle damage within weeks. Given it back, both reversed.

  • 07

    NAC

    400 mg

    N-acetyl-L-cysteine, film coated, minimum 98%

    • STRESS
    • SKIN
    • DIGESTION
    • EYES
    • LIVER
    ₹0.90 a sachet

    Why it is here

    The rate limiting building block of glutathione. Your body cannot make more glutathione than it has cysteine, and NAC is how you give it cysteine safely.

    The trial

    Kumar 2023, Journals of Gerontology. NAC with glycine for sixteen weeks restored glutathione, lowered oxidative stress and inflammation, and improved physical function in adults aged 61 to 80.

  • 08

    Ashwagandha

    300 mg

    Withania somnifera root only, minimum 2.5% withanolides

    • ENERGY
    • STRESS
    ₹0.80 a sachet

    Why it is here

    Lower cortisol and easier sleep, with no sedation and no tolerance, which is why it can be taken every day where rhodiola and valerian cannot.

    The trial

    Chandrasekhar 2012, Indian Journal of Psychological Medicine. Sixty days of root extract cut perceived stress scores by 44 per cent and serum cortisol by 28 per cent.

  • 09

    Curcumin

    300 mg, 90 mg curcuminoids

    Water soluble, minimum 30% total curcuminoids

    • SKIN
    • JOINTS
    • HEART
    • LIVER
    ₹2.20 a sachet

    Why it is here

    One of the four ingredients that cover the several paths a joint fire burns along, and a background anti-inflammatory for skin, heart and liver. Water soluble, so no piperine is needed to absorb it.

    The trial

    Cox 2015, Journal of Psychopharmacology. A high absorption curcumin improved working memory and mood in healthy adults aged 60 to 85, within an hour and again at four weeks.

  • 10

    Acacia Fibre

    250 mg

    Acacia senegal gum arabic

    • CARRIER
    ₹0.12 a sachet

    Why it is here

    It carries the actives so every sachet is the same sachet. It claims nothing of its own.

    The trial

    None claimed. Prebiotic effects of gum arabic appear at 5 to 10 g a day, twenty times this amount, and we will not borrow them.

  • 11

    Boswellia

    250 mg

    Boswellia serrata, AKBA enriched extract, 20% AKBA

    • DIGESTION
    • JOINTS
    ₹0.90 a sachet

    Why it is here

    It blocks the inflammatory path turmeric does not reach, 5-LOX, and it is the fastest acting of the four joint ingredients.

    The trial

    Sengupta 2008, Arthritis Research and Therapy. Ninety days of an AKBA enriched extract reduced knee pain, improved function, and lowered MMP-3, the enzyme that degrades cartilage, in joint fluid.

  • 12

    Milk Thistle

    250 mg, 200 mg silymarin

    Silybum marianum, 80% silymarin

    • LIVER
    ₹0.70 a sachet

    Why it is here

    The liver's traditional protective herb, with a clean safety record over decades of daily use and a stabilising effect on liver cell membranes.

    The trial

    Silymarin meta-analysis 2017, Medicine. In people with fatty liver, silymarin lowered ALT and AST more than control.

  • 13

    American Ginseng

    200 mg

    Panax quinquefolius root, minimum 10% ginsenosides

    • ENERGY
    • BRAIN
    ₹2.00 a sachet

    Why it is here

    Working memory and calm alertness in the same dose, without the stimulation, and with fewer drug interactions in the literature than the Asian species.

    The trial

    Scholey 2010, Psychopharmacology. American ginseng improved working memory and self rated calmness in healthy adults within hours.

  • 14

    Bacopa monnieri

    200 mg, 80 mg bacosides

    Whole plant, minimum 40% bacosides

    • BRAIN
    ₹0.90 a sachet

    Why it is here

    The Ayurvedic memory herb with the best controlled evidence, and one of the few that works with daily use rather than fading.

    The trial

    Kongkeaw 2014, Journal of Ethnopharmacology. Nine trials pooled: bacopa improved attention and speed of processing, and took twelve weeks to do it.

  • 15

    Sunflower Lecithin

    200 mg

    Minimum 95% phospholipids

    • CARRIER
    ₹0.20 a sachet

    Why it is here

    It lets the fat soluble actives disperse in water so they can be absorbed. Without it, several of the actives above would sit on top of the glass.

    The trial

    None claimed. It is a carrier.

  • 16

    L-Theanine

    100 mg

    Minimum 98% L-isomer

    • BRAIN
    • STRESS
    ₹1.10 a sachet

    Why it is here

    The compound in green tea that makes caffeine feel smooth, taken without the caffeine. Calm attention, not sedation.

    The trial

    Hidese 2019, Nutrients. Four weeks of L-theanine reduced stress related symptoms and improved sleep and cognition in healthy adults.

  • 17

    CoQ10

    100 mg

    Ubiquinone, minimum 99%

    • ENERGY
    • HEART
    ₹4.00 a sachet

    Why it is here

    The middle of the energy production line, and the one part of it your body makes less of every year. Heart tissue holds more of it than anything else in the body.

    The trial

    Mortensen 2014, JACC Heart Failure, the Q-SYMBIO trial. Two years of CoQ10 on top of standard treatment roughly halved major cardiac events in heart failure patients.

  • 18

    Ginger

    100 mg

    Zingiber officinale, water soluble, minimum 5% gingerols

    • DIGESTION
    • JOINTS
    ₹0.35 a sachet

    Why it is here

    Faster stomach emptying after a meal, and the third of the four joint ingredients.

    The trial

    Wu 2008, European Journal of Gastroenterology and Hepatology. Ginger sped up stomach emptying in healthy volunteers.

  • 19

    Lutein and Zeaxanthin

    100 mg

    Water soluble, 5:1 ratio

    • EYES
    ₹2.50 a sachet

    Why it is here

    Together they form the pigment at the centre of the retina that filters the blue light which damages it. Zeaxanthin holds the centre, lutein the ring around it, and the filter is only complete with both.

    The trial

    Stringham 2017, Foods. Six months of the two carotenoids gave people spending long hours on screens less eye strain, less eye fatigue and fewer headaches.

  • 20

    Vitamin C

    65 mg

    Sodium ascorbate

    • SKIN
    • IMMUNITY
    • JOINTS
    • EYES
    • HEART
    ₹0.06 a sachet

    Why it is here

    The enzyme that sets collagen cannot run without it, and it hands vitamin E back its charge in the antioxidant relay. Indian cooking destroys much of the vitamin C on the plate.

    The trial

    Hemilä 2013, Cochrane Database of Systematic Reviews. Taken every day, vitamin C shortened adults' colds by about eight per cent.

  • 21

    Zinc Citrate

    35 mg, 10.5 mg zinc

    30% elemental

    • SKIN
    • DIGESTION
    • IMMUNITY
    • EYES
    ₹0.08 a sachet

    Why it is here

    Immune cells, skin renewal and the retina all need it, and the phytates in dal lock up most of the zinc dal contains. Citrate, because it absorbs as well as gluconate and better than oxide (Wegmüller 2014).

    The trial

    Prasad 2007, American Journal of Clinical Nutrition. A year of zinc cut infection rates and oxidative stress markers in older adults.

  • 22

    Digestive Enzyme Blend

    25 mg

    Lactase, protease and amylase

    • DIGESTION
    ₹0.60 a sachet

    Why it is here

    They cut milk sugar, protein and starch into pieces small enough to absorb, which matters most after a heavy Indian lunch.

    The trial

    None claimed. There is no outcome trial on this blend, and we will not quote one that does not exist.

  • 23

    Bacillus coagulans

    20 mg, 2 billion CFU

    MTCC 5856, spore form

    • DIGESTION
    • IMMUNITY
    ₹1.30 a sachet

    Why it is here

    A spore forming probiotic, so it survives stomach acid and reaches the intestine alive, at the exact strain and count that was trialled.

    The trial

    Majeed 2016, Nutrition Journal. Ninety days of this strain reduced bloating, abdominal pain and stool frequency in people with irritable bowels.

  • 24

    Saffron

    15 mg

    Crocus sativus stigma, 1% safranal

    • BRAIN
    • STRESS
    • EYES
    ₹3.00 a sachet

    Why it is here

    Sleep quality and steadier mood, with the eye's carotenoids as a bonus; crocin is one of them.

    The trial

    Pachikian 2021, Nutrients. Six weeks of saffron extract in the evening improved sleep quality against placebo.

  • 25

    Astaxanthin

    12 mg

    Natural astaxanthin

    • BRAIN
    • SKIN
    • EYES
    ₹3.25 a sachet

    Why it is here

    The carotenoid that gives salmon its colour, and the strongest of the antioxidants that sit inside a cell membrane. Skin, eyes and brain all get it.

    The trial

    Tominaga 2017, Journal of Clinical Biochemistry and Nutrition. Sixteen weeks of astaxanthin protected skin elasticity and moisture against seasonal decline.

  • 26

    Vitamin E

    10 mg

    Natural mixed tocopherols

    • SKIN
    • EYES
    • HEART
    ₹0.10 a sachet

    Why it is here

    The antioxidant that works inside the fat layer, where water based protection cannot reach. It takes the hit, and vitamin C recharges it.

    The trial

    None at this dose, and the high dose trials are a different product. Traber 2007, Free Radical Biology and Medicine, is the reference for how it works.

  • 27

    Copper Gluconate

    6 mg, 0.84 mg copper

    14% elemental

    • ENERGY
    • SKIN
    • IMMUNITY
    • JOINTS
    • EYES
    • HEART
    ₹0.03 a sachet

    Why it is here

    To prevent a harm rather than deliver a benefit. Zinc taken for years without matching copper causes copper deficiency, and copper also closes the energy production line and cross links collagen. Copper is in this formula because zinc is.

    The trial

    None claimed. This one is textbook mineral balance, not a trial claim.

  • 28

    Vitamin D3

    600 IU, 15 mcg

    Cholecalciferol from lichen, 6 mg of a 100 IU per mg premix

    • IMMUNITY
    ₹0.08 a sachet

    Why it is here

    It switches immune cells on, once magnesium has converted it. Same sun, less made every year, and most Indians test low despite the sun.

    The trial

    Jolliffe 2021, Lancet Diabetes and Endocrinology. Pooled data from 43 trials: daily vitamin D gave the clearest protection against respiratory infections.

  • 29

    Vitamin B6

    1.9 mg

    Pyridoxal-5-phosphate

    • ENERGY
    • BRAIN
    • STRESS
    • HEART
    ₹0.05 a sachet

    Why it is here

    The co-factor for the route that clears homocysteine after meals, for building red blood cells, and for the enzymes that make serotonin and dopamine. In the active form, so no conversion is needed.

    The trial

    None at this dose, and we will not borrow one from a higher dose.

  • 30

    Vitamin B2

    1.9 mg

    Riboflavin

    • ENERGY
    • BRAIN
    • STRESS
    • SKIN
    • DIGESTION
    • EYES
    • HEART
    • LIVER
    ₹0.05 a sachet

    Why it is here

    The switch that starts the energy production line, converts folate into its usable form, keeps the B12 enzyme running, and recharges glutathione after every use. Eight pillars, the most of any active. It also turns urine bright yellow; that is riboflavin, not a problem.

    The trial

    McNulty 2006, Circulation. Twelve weeks of riboflavin lowered homocysteine by 22 per cent in people carrying the MTHFR variant, whose folate enzyme runs slow without it.

  • 31

    Folic Acid

    220 mcg

    Pteroylglutamic acid

    • ENERGY
    • BRAIN
    • STRESS
    • HEART
    ₹0.01 a sachet

    Why it is here

    It carries the tag that clears homocysteine, and red blood cells cannot be built without it. Riboflavin, above, is what switches it into the form your body uses.

    The trial

    Homocysteine Lowering Trialists 2005, American Journal of Clinical Nutrition. Pooled trials: 0.2 mg of folic acid a day lowered homocysteine by about 13 per cent, 0.4 mg by about 20, and doses above that added little.

  • 32

    Vitamin B12

    2.5 mcg

    Hydroxocobalamin

    • ENERGY
    • BRAIN
    • STRESS
    • HEART
    ₹0.12 a sachet

    Why it is here

    Almost no plant food has it, and Indian studies find between half and seven in ten vegetarian adults running low. It hands homocysteine over for clearing, and red blood cells need it.

    The trial

    Deshmukh 2010, European Journal of Clinical Nutrition. In Pune, a year of low dose B12 lowered homocysteine by a mean 5.9 μmol/L in 300 adults; a five times larger dose did no better.

What the 32 cost us

  • 32 Actives
  • ₹38.30 A sachet
  • ₹2,298 60 sachets

Flavour and sweetener, 907 mg, extra and declared in full on the pack. None of it is here to do anything to your body.